Publications
Cell2Cure has a license from Rigshospitalet, Copenhagen, to a proprietary ASC production technology platform. The ASC technology has an extensive publication background both clinically and preclinically.
Production and preclinical
Optimizing an immunomodulatory potency assay for Mesenchymal Stromal Cell.
Hansen SB, Højgaard LD, Kastrup J, Ekblond A, Follin B, Juhl M. Optimizing an immunomodulatory potency assay for Mesenchymal Stromal Cell. Front Immunol. 2022 Dec 12;13:1085312. doi: 10.3389/fimmu.2022.1085312. PMID: 36578497; PMCID: PMC9791065.
Functional in vitro models of the inhibitory effect of adipose tissue-derived stromal cells on lymphocyte proliferation: Improved sensitivity and quantification through flow cytometric analysis.
Juhl M, Follin B, Christensen JP, Kastrup J, Ekblond A. Functional in vitro models of the inhibitory effect of adipose tissue-derived stromal cells on lymphocyte proliferation: Improved sensitivity and quantification through flow cytometric analysis. J Immunol Methods. 2022 Nov;510:113360. doi: 10.1016/j.jim.2022.113360. Epub 2022 Sep 18. PMID: 36130659.
GMP Compliant Production of a Cryopreserved Adipose-Derived Stromal Cell Product for Feasible and Allogeneic Clinical Use.
Haack-Sørensen M, Johansen EM, Højgaard LD, Kastrup J, Ekblond A.Stem Cells Int. 2022 Jun 20;2022:4664917. doi: 10.1155/2022/4664917.
Adipose-derived stromal cells increase the formation of collagens through paracrine and juxtacrine mechanisms in a fibroblast co-culture model utilizing macromolecular crowding.
Søndergaard RH, Højgaard LD, Reese-Petersen AL, Hoeeg C, Mathiasen AB, Haack-Sørensen M, Follin B, Genovese F, Kastrup J, Juhl M, Ekblond A.Stem Cell Res Ther. 2022 Jun 11;13(1):250. doi: 10.1186/s13287-022-02923-y.
The Initial Cardiac Tissue Response to Cryopreserved Allogeneic Adipose Tissue-Derived Mesenchymal Stromal Cells in Rats with Chronic Ischemic Cardiomyopathy.
Follin B, Hoeeg C, Højgaard LD, Juhl M, Lund KB, Døssing KBV, Bentsen S, Hunter I, Nielsen CH, Ripa RS, Kastrup J, Ekblond A, Kjaer A.Int J Mol Sci. 2021 Oct 29;22(21):11758. doi: 10.3390/ijms222111758.
Adipose Tissue-Derived Stromal Cells Induce a Highly Trophic Environment While Reducing Maturation of Monocyte-Derived Dendritic Cells.
Juhl M, Follin B, Gad M, Larsen J, Kastrup J, Ekblond A.Stem Cells Int. 2020 Oct 26;2020:8868909. doi: 10.1155/2020/8868909.
Development of large-scale manufacturing of adipose-derived stromal cells for clinical applications using bioreactors and human platelet lysate.
Haack-Sørensen M, Juhl M, Follin B, Harary Søndergaard R, Kirchhoff M, Kastrup J, Ekblond A.Scand J Clin Lab Invest. 2018 Jul;78(4):293-300. doi: 10.1080/00365513.2018.1462082.
Senescence and quiescence in adipose-derived stromal cells: Effects of human platelet lysate, fetal bovine serum and hypoxia.
Søndergaard RH, Follin B, Lund LD, Juhl M, Ekblond A, Kastrup J, Haack-Sørensen M.Cytotherapy. 2017 Jan;19(1):95-106. doi: 10.1016/j.jcyt.2016.09.006.
Culture expansion of adipose derived stromal cells. A closed automated Quantum Cell Expansion System compared with manual flask-based culture.
Haack-Sørensen M, Follin B, Juhl M, Brorsen SK, Søndergaard RH, Kastrup J, Ekblond A.J Transl Med. 2016 Nov 16;14(1):319. doi: 10.1186/s12967-016-1080-9.
Comparison of clinical grade human platelet lysates for cultivation of mesenchymal stromal cells from bone marrow and adipose tissue.
Juhl M, Tratwal J, Follin B, Søndergaard RH, Kirchhoff M, Ekblond A, Kastrup J, Haack-Sørensen M.Scand J Clin Lab Invest. 2016;76(2):93-104. doi: 10.3109/00365513.2015.1099723.
Clinical
Effect of allogenic adipose tissue-derived mesenchymal stromal cell treatment in chronic ischaemic heart failure with reduced ejection fraction – the SCIENCE trial.
Qayyum AA, van Klarenbosch B, Frljak S, Cerar A, Poglajen G, Traxler-Weidenauer D, Nadrowski P, Paitazoglou C, Vrtovec B, Bergmann MW, Chamuleau SAJ, Wojakowski W, Gyöngyösi M, Kraaijeveld A, Hansen KS, Vrangbaek K, Jørgensen E, Helqvist S, Joshi FR, Johansen EM, Follin B, Juhl M, Højgaard LD, Mathiasen AB, Ekblond A, Haack-Sørensen M, Kastrup J; SCIENCE Investigators. Effect of allogeneic adipose tissue-derived mesenchymal stromal cell treatment in chronic ischaemic heart failure with reduced ejection fraction – the SCIENCE trial. Eur J Heart Fail. 2023 Jan 16. doi: 10.1002/ejhf.2772. Epub ahead of print. PMID: 36644821.
Danish phase II trial using adipose tissue derived mesenchymal stromal cells for patients with ischaemic heart failure.
Qayyum AA, Mouridsen M, Nilsson B, Gustafsson I, Schou M, Nielsen OW, Hove JD, Mathiasen AB, Jørgensen E, Helqvist S, Joshi FR, Johansen EM, Follin B, Juhl M, Højgaard LD, Haack-Sørensen M, Ekblond A, Kastrup J. Danish phase II trial using adipose tissue derived mesenchymal stromal cells for patients with ischaemic heart failure. ESC Heart Fail. 2023 Jan 13. doi: 10.1002/ehf2.14281. Epub ahead of print. PMID: 36638837.
Intraglandular mesenchymal stem cell treatment induces changes in the salivary proteome of irradiated patients.
Lynggaard CD, Jersie-Christensen R, Juhl M, Jensen SB, Grønhøj C, Melchiors J, Jacobsen S, Møller-Hansen M, Herly M, Ekblond A, Kastrup J, Fischer-Nielsen A, Belstrøm D, von Buchwald C. Intraglandular mesenchymal stem cell treatment induces changes in the salivary proteome of irradiated patients. Commun Med (Lond). 2022 Dec 10;2(1):160. doi: 10.1038/s43856-022-00223-3. PMID: 36496530; PMCID: PMC9735277.
Intraglandular Off-the-Shelf Allogeneic Mesenchymal Stem Cell Treatment in Patients with Radiation-Induced Xerostomia: A Safety Study (MESRIX-II).
Lynggaard CD, Grønhøj C, Christensen R, Fischer-Nielsen A, Melchiors J, Specht L, Andersen E, Mortensen J, Oturai P, Barfod GH, Haastrup EK, Møller-Hansen M, Haack-Sørensen M, Ekblond A, Kastrup J, Jensen SB, von Buchwald C.Stem Cells Transl Med. 2022 May 27;11(5):478-489. doi: 10.1093/stcltm/szac011.
Safety and feasibility of mesenchymal stem cell therapy in patients with aqueous deficient dry eye disease.
Møller-Hansen M, Larsen AC, Toft PB, Lynggaard CD, Schwartz C, Bruunsgaard H, Haack-Sørensen M, Ekblond A, Kastrup J, Heegaard S.Ocul Surf. 2021 Jan;19:43-52. doi: 10.1016/j.jtos.2020.11.013.
Rationale and design of the European multicentre study on Stem Cell therapy in IschEmic Non-treatable Cardiac diseasE (SCIENCE).
Paitazoglou C, Bergmann MW, Vrtovec B, Chamuleau SAJ, van Klarenbosch B, Wojakowski W, Michalewska-Włudarczyk A, Gyöngyösi M, Ekblond A, Haack-Sørensen M, Jaquet K, Vrangbaek K, Kastrup J; SCIENCE Investigators.Eur J Heart Fail. 2019 Aug;21(8):1032-1041. doi: 10.1002/ejhf.1412.
Cryopreserved Off-the-Shelf Allogeneic Adipose-Derived Stromal Cells for Therapy in Patients with Ischemic Heart Disease and Heart Failure-A Safety Study.
Kastrup J, Haack-Sørensen M, Juhl M, Harary Søndergaard R, Follin B, Drozd Lund L, Mønsted Johansen E, Ali Qayyum A, Bruun Mathiasen A, Jørgensen E, Helqvist S, Jørgen Elberg J, Bruunsgaard H, Ekblond A.Stem Cells Transl Med. 2017 Nov;6(11):1963-1971. doi: 10.1002/sctm.17-0040.
Rationale and Design of the First Double-Blind, Placebo-Controlled Trial with Allogeneic Adipose Tissue-Derived Stromal Cell Therapy in Patients with Ischemic Heart Failure: A Phase II Danish Multicentre Study.
Kastrup J, Schou M, Gustafsson I, Nielsen OW, Møgelvang R, Kofoed KF, Kragelund C, Hove JD, Fabricius-Bjerre A, Heitman M, Haack-Sørensen M, Lund LD, Johansen EM, Qayyum AA, Mathiasen AB, Ekblond A.Stem Cells Int. 2017;2017:8506370. doi: 10.1155/2017/8506370.
Cell2Cure has a license from Rigshospitalet, Copenhagen, to a proprietary ASC production technology platform. The ASC technology has an extensive publication background both clinically and preclinically.
CONTACT US
Innobooster grant
SME Instrument grant
This project has received funding from the European Union’s SME Instrument, Horizon 2020 research and innovation programme under grant agreement No [H2020-SMEINST-868284_PRO-SCT]
Grand Solution Grant
Cell2Cure, as part of the consortium Clinical Stem cell Innovation and production Center, CSIC, receives funding from Innovation Fund Denmark.